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YMC-Pack Diol-SEC

작성자
예일테크
작성일
2008.06.17
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내용

YMC-Pack Diol-SEC

 

◈ Product Information

YMC-Pack Diol-SEC is a size exclusion chromatography column utilizing a silica gel base. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands. Diol-60 is the most suitable for separation of peptides of oligosaccharides whose molecular weights are 10,000 or less.

 

 ► Point  

   • Utilizes silica gel base with high resistance to pressure  

   • Low-cost gel filtration column  

   • Useful for molecular weight determination of proteins and sugars  

   • Minimal secondary interactions

 

 ► Specification  

   - Particle size :5㎛  

   - Pore size :60A, 120A, 200A, 300A  

   - Usable pH range :5.0-7.5

 

 Calibration curves of proteins obtained using columns with different pore sizes

 

 

1. Glutamate dehydrogenase

2. Lactate dehydrogenase

3. Enolase

4. Adenylate kinase

5. Cytochrome c

 

• Column :YMC-Pack Diol-SEC 500 x 8.0 mmI.D.

• Eluent :0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl

• Flow rate :0.7 mL/min

• Temperature :ambient

• Detection :UV at 280 nm

 

Diol-120,Diol-200,and Diol-300 are suitable for the separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.

 

 For separation of proteins with molecular weights of 10,000 to several hundred thousands

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1.Glutamate dehydrogenase(MW 290,000)

2. Lactate dehydrogenase(MW 142,000)

3. Enolase(MW 67,000)

4. Adenylate kinase(MW 32,000)

5. Cytochrome c(MW 12,400)

 

• Column :YMC-Pack Diol-SEC 500 x 8.0 mmI.D.

• Eluent :0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl

• Flow rate :0.7 mL/min

• Temperature :ambient

• Detection :UV at 280 nm

 

 For separation of peptides with molecular weights of 10,000 or less

 

 

1.Insulin (Bovine)

2.Neurotensin

3.Angiotensin-II

4.Glycine

 

• Column :YMC-Pack Diol-60 500 x 8.0 mmI.D.

• Eluent :0.1M KH2PO4-K2HPO4(pH 7.0) containing 0.2MNaCl /acetonitrile(70/30)

• Flow rate :0.7 mL/min

• Temperature :ambient

• Detection :UV at 215 nm

 

 

1. Myoglobin-17,000(MW)

2. Ribonuclease A-13,700(MW)

3. Cytochrome c-12,400(MW)

4. Insulin (Bovine)-5,700(MW)

5. Insulin B chain-3,496(MW)

6. α-Mating factor-1,684(MW)

7. Neurotensin-1,672(MW)

8. Angiotensin-I-1,296(MW)

9. CCK-octapeptide-1,143(MW)

10. Bradykinin-1,060(MW)

11. Angiotensin-II-1,040(MW)

12. Oxytocin-1,007(MW)

13. Angiotensin-III-931(MW)

14. Met-enkephalin-573(MW)

15. Leu-enkephalin-555(MW)

16. Tetraglycine-246(MW)

17. Glycine-75(MW)

 

 For molecular weight determination of sugars

 

 

 

For separation or molecular weight determination of neutral sugars and water-soluble high-molecular-weight compounds,Diol-60,Diol-120,Diol-200,and Diol-300 are useful individually or in combination.

 

◈ Ordering Information

YMC-Pack Diol-SEC Analysis Columns(Stainless Columns)

Packing material

Column size Inner diameter x length(mm)

Product Number

Diol-60

8.0×300

DL06S05-3008WT

8.0×500

DL06S05-5008WT

Diol-120

8.0×300

DL12S05-3008WT

8.0×500

DL12S05-5008WT

Diol-200

8.0×300

DL20S05-3008WT

8.0×500

DL20S05-5008WT

Diol-300

8.0×300

DL30S05-3008WT

8.0×500

DL30S05-5008WT

YMC-Pack Diol-SEC Guard Columns(Stainless Columns)

Packing material

Column size Inner diameter x length(mm)

Product Number

Diol-60

8.0×30

DL06S05-0308WTG

Diol-120

8.0×30

DL12S05-0308WTG

Diol-200

8.0×30

DL20S05-0308WTG

Diol-300

8.0×30

DL30S05-0308WTG

YMC-Pack Diol-SEC Analysis Columns(Glass Columns)

Packing material

Column size Inner diameter x length(mm)

Product Number

Diol-60

8.0×300

DL06S05-3008FG

8.0×500

DL06S05-5008FG

Diol-120

8.0×300

DL12S05-3008FG

8.0×500

DL12S05-5008FG

Diol-200

8.0×300

DL20S05-3008FG

8.0×500

DL20S05-5008FG

Diol-300

8.0×300

DL30S05-3008FG

8.0×500

DL30S05-5008FG

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